Nuclease is the term used for an enzyme that cleaves nucleic acids. Nucleases belong to the enzyme class hydrolases and perform a specific role. Nuclease has the role of cleaving the phosphodiester bonds between nucleotides of nucleic acids and causing single and double-stranded breaks in their target molecules.
UltraNuclease（Benzonase Nuclease）produced by Hizyme can cleave all kinds of DNA and RNA variants into fragments that comprise < 8 soluble base pairs. This leads to an utmost minimum of nucleic acid load, and hence to an acceptable lower viscosity of the process fluid.
UltraNuclease（Benzonase Nuclease）has an exceptionally high level of specific activity for nucleic acids without any detectable proteolytic activity. It is effective in a wide range of operating conditions and applications, including the following:purification of viral vaccines, viral vectors for vaccine, cell and gene therapy, and oncolytic viruses, removing DNA/RNA from proteins and other biologicals reduction of viscosity caused by nucleic acids sample preparation in electrophoresis and chromatography prevention of cell clumping.
The degree of purity for the enzyme is above 99% .Using the method of precipitate the undigested DNA using perchloric acid to determine the enzyme activity，enzyme activity above 700 U/μL，specific activity above 1*10^6U/mg.The optimum pH and the optimum temperature of this product were about 7.5-8.0 and 37 ℃ .More than 50 % residual enzyme activity at 57 °C,more than 30 % residual enzyme activity at pH=4.0.Therefore, it is competitive in the same function products